| Solution Information | help | |
| Enzyme: | Guanyl-specific ribonuclease T1 | |
| inhibitor: | BDBM54791 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose-response curves for 20 compounds that demonstrated differential activities in a set of previous experiments entitled, "Fluorescence polarization-based confirmation biochemical high throughput screening assay to identify inhibitors of tRNA 2'-phosphotransferase (TPT1)" (AID 2149) and, "Fluorescence polarization-based counterscreen assay for inhibitors of tRNA 2'-phosphotransferase (TPT1): biochemical high throughput screening assay to identify inhibitors of RNAse T1" (AID 2153). Compounds with a greater activity in the TPT1 assay compared to the RNase T1 counterscreen were selected and sorted by their distance (D) from a state where percent inhibition in both assays were equal according to the following equation: D = square root ( squared ( "%inhibition TPT1 assay" - "%inhibition RNase T1 counterscreen" ) / 2 ). The 20 compounds with the highest D value were selected for dose-response determinations. This fluore | |
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